Composite

Part:BBa_K3286009:Design

Designed by: Paul Alexander Niederau   Group: iGEM19_Wageningen_UR   (2019-10-16)


AcrIIA4 expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The AcrIIA4 expression was adapted by the use of different RBS sites. The deeper analysis on varying Acr levels on inhibitory activity of dCas9 were assessed here: https://parts.igem.org/Part:BBa_K3286010


Source

AcrIIA4 was ordered as a BioBrick from Integrated DNA Technologies.

References